AGBL offers a broad selection of research products and services for cell biology, molecular biology, drug discovery and applied research from Lonza, an integrated solutions provider of products that creates value along the Healthcare Continuum®, based in the Swiss Alps.
Highlighted below are some of the tools that will provide life science researchers with the tools they need to develop and test therapeutics, from basic research to final product release. Lonza’s Bioscience products and services range from cell culture and discovery technologies for research, to quality control tests and software for biomanufacturing.
In the following sections, Lonza outlines the methodological principles of in vitro stem cell culture and its benefits and challenges, and how their innovative NucleofectorTM Technology can help to overcome the challenges of transfecting stem cells. Also reviewed are the different types of stem cells offered, including iPSCs and adult stem cells such as mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs). This will assist in identification of which types are best suited to various applications. Lastly, the different applications of stem cell culture, including in disease research and therapy, to elucidate the importance of stem cells in clinical and research progression, is explained.
In the following sections we discuss key topics in cell culture, such as animal and human cell cultures, stem cells, adherent cell cultures, and suspension cell cultures. We also look at different types of cell culture media, buffers and reagents, and how mycoplasma contamination can negatively affect your cell cultures.
Know Your Enemy – Mycoplasma Contamination in Cell Culture
Watch this webinar on mycoplasma testing and learn about potential impacts of a contamination on cell cultures and how you can easily uncover and remove it.
Transfection is generally defined as the process of introducing DNA, RNA or proteins into cells to influence their genotype or phenotype. This includes introducing new genes to study or leverage their function, as well as using other constructs to indirectly influence endogenous gene expression or other cellular processes. Although less common, protein transfection is also used, either to generate a more rapid response or to facilitate certain applications (for example, CRISPR for genome editing).
Transfection has many applications. Besides basic research, these include gene therapy, immunotherapy and induced pluripotent stem cell generation. Meanwhile, recombinant protein expression also enables a wide range of functional, genetic mapping and biochemical characterization studies, in addition to bioproduction applications. Different types of cells can be transfected (such as primary cells, cell lines, and stem cells), making this a powerful tool for use across the life sciences.
Lonza offers a range of products for molecular biology applications including electrophoresis of DNA and RNA, PFGE for large fragment analysis, PCR fragment analysis, DNA and RNA recovery and cloning, and Northern and Southern blotting, as well as Protein electrophoresis. Agarose options include agarose appropriate for special applications such as viral plaque assays, cell culture, cDNA library expansion, comet assays, and more.
Lonza solutions for bacterial endotoxin testing are all about scientific discoveries that help define the way endotoxin tests are performed, and how these breakthroughs can impact regulatory guidelines in our industry, and help ensure the quality, efficiency, and sustainability of endotoxin testing, for your products and your patients.
QC InsiderTM Toolbox
Endotoxin Expertise At Your FingertipsTM
QC InsiderTM Toolbox is packed with comprehensive support tools that will help you perform the bacterial endotoxins test. It has been created by QC experts who face many of the same regulated manufacturing challenges as you do.